| 质粒名称: | pBTK562 (pDS-GFP) |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 183129 |
| 存储实验室: | Jeffrey Barrick |
|
载体骨架基本信息 |
|
| 载体名称: | RSF1010 |
| 空载体大小: | 4038 bp |
| 完整质粒大小: | 4989 bp |
| 载体抗性: | Spectinomycin, 50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | GFPmut3 (no RBS) |
| 物种来源: | Synthetic |
| 插入大小: | 717 bp |
|
文献引用方法 |
|
| 材料和方法部分: | pBTK562 (pDS-GFP) was a gift from Jeffrey Barrick (Addgene plasmid # 183129 ; http://n2t.net/addgene:183129 ; RRID:Addgene_183129) |
| 参考文献部分: | Engineered symbionts activate honey bee immunity and limit pathogens. Leonard SP, Powell JE, Perutka J, Geng P, Heckmann LC, Horak RD, Davies BW, Ellington AD, Barrick JE, Moran NA. Science. 2020 Jan 31;367(6477):573-576. doi: 10.1126/science.aax9039. Epub 2020 Jan 30. 10.1126/science.aax9039 PubMed 32001655 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
183129质粒图谱.png)
载体简介
载体序列
LOCUS pBTK562 (pDS-GFP) 8101 bp ds-DNA circular SYN 24-JUN-2022
DEFINITION Control plasmid that expresses off-target dsRNA.
ACCESSION .
VERSION .
KEYWORDS pBTK562 (pDS-GFP)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8101)
AUTHORS Leonard SP, Powell JE, Perutka J, Geng P, Heckmann LC, Horak RD,
Davies BW, Ellington AD, Barrick JE, Moran NA
TITLE Engineered symbionts activate honey bee immunity and limit
pathogens.
JOURNAL Science. 2020 Jan 31;367(6477):573-576. doi:
10.1126/science.aax9039. Epub 2020 Jan 30.
PUBMED 32001655
REFERENCE 2 (bases 1 to 8101)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 24, 2022 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8101
/organism="synthetic DNA construct"
/mol_type="other DNA"
oriT 2210..2297
/label=RSF1010 oriT
/note="origin of transfer of the broad-host-range plasmid
RSF1010 (Scholz et al., 1989)"
rep_origin 2639..3033
/label=RSF1010 oriV
/note="replication origin of the broad-host-range plasmid
RSF1010; requires the RSF1010 RepA/B/C proteins for
replication (Scholz et al., 1989)"
CDS complement(4742..5533)
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
CDS complement(6751..7590)
/codon_start=1
/gene="repA"
/product="replication protein A of the broad-host-range
plasmid RSF1010 (Scholz et al., 1989)"
/label=RSF1010 RepA
/translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
CDS complement(8101..971)
/codon_start=1
/gene="repB"
/product="replication protein B of the broad-host-range
plasmid RSF1010 (Scholz et al., 1989)"
/label=RSF1010 RepB
/translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
ORIGIN
1 tacatgctga aatctggccc gcccctgtcc atgcctcgct ggcggggtgc cggtgcccgt
61 gccagctcgg cccgcgcaag ctggacgctg ggcagaccca tgaccttgct gacggtgcgc
121 tcgatgtaat ccgcttcgtg gccgggcttg cgctctgcca gcgctgggct ggcctcggcc
181 atggccttgc cgatttcctc ggcactgcgg ccccggctgg ccagcttctg cgcggcgata
241 aagtcgcact tgctgaggtc atcaccgaag cgcttgacca gcccggccat ctcgctgcgg
301 tactcgtcca gcgccgtgcg ccggtggcgg ctaagctgcc gctcgggcag ttcgaggctg
361 gccagcctgc gggccttctc ctgctgccgc tgggcctgct cgatctgctg gccagcctgc
421 tgcaccagcg ccgggccagc ggtggcggtc ttgcccttgg attcacgcag cagcacccac
481 ggctgataac cggcgcgggt ggtgtgcttg tccttgcggt tggtgaagcc cgccaagcgg
541 ccatagtggc ggctgtcggc gctggccggg tcggcgtcgt actcgctggc cagcgtccgg
601 gcaatctgcc cccgaagttc accgcctgcg gcgtcggcca ccttgaccca tgcctgatag
661 ttcttcgggc tggtttccac taccagggca ggctcccggc cctcggcttt catgtcatcc
721 aggtcaaact cgctgaggtc gtccaccagc accagaccat gccgctcctg ctcggcgggc
781 ctgatataca cgtcattgcc ctgggcattc atccgcttga gccatggcgt gttctggagc
841 acttcggcgg ctgaccattc ccggttcatc atctggccgg tggtggcgtc cctgacgccg
901 atatcgaagc gctcacagcc catggccttg agctgtcggc ctatggcctg caaagtcctg
961 tcgttcttca tcgggccacc aagcgcagcc agatcgagcc gtcctcggtt gtcagtggcg
1021 tcaggtcgag caagagcaac gatgcgatca gcagcaccac cgtaggcatc atggaagcca
1081 gcatcacggt tagccatagc ttccagtgcc acccccgcga cgcgctccgg gcgctctgcg
1141 cggcgctgct cacctcggcg gctacctccc gcaactcttt ggccagctcc acccatgccg
1201 cccctgtctg gcgctgggct ttcagccact ccgccgcctg cgcctcgctg gcctgctggg
1261 tctggctcat gacctgccgg gcttcgtcgg ccagtgtcgc catgctctgg gccagcggtt
1321 cgatctgctc cgctaactcg ttgatgcctc tggatttctt cactctgtcg attgcgttca
1381 tggtctattg cctcccggta ttcctgtaag tcgatgatct gggcgttggc ggtgtcgatg
1441 ttcagggcca cgtctgcccg gtcggtgcgg atgccccggc cttccatctc caccacgttc
1501 ggccccaggt gaacaccggg caggcgctcg atgccctgcg cctcaagtgt tctgtggtca
1561 atgcgggcgt cgtggccagc ccgctctaat gcccggttgg catggtcggc ccatgcctcg
1621 cgggtctgct caagccatgc cttgggcttg agcgcttcgg tcttctgtgc cccgcccttc
1681 tccggggtct tgccgttgta ccgcttgaac cactgagcgg cgggccgctc gatgccgtca
1741 ttgatccgct cggagatcat caggtggcag tgcgggttct cgccgccacc ggcatggatg
1801 gccagcgtat acggcaggcg ctcggcaccg gtcaggtgct gggcgaactc ggacgccagc
1861 gccttctgct ggtcgagggt cagctcgacc ggcagggcaa attcgacctc cttgaacagc
1921 cgcccattgg cgcgttcata caggtcggca gcatcccagt agtcggcggg ccgctcgacg
1981 aactccggca tgtgcccgga ttcggcgtgc aagacttcat ccatgtcgcg ggcatacttg
2041 ccttcgcgct ggatgtagtc ggccttggcc ctggccgatt ggccgcccga cctgctgccg
2101 gttttcgccg taaggtgata aatcgccatg ctgcctcgct gttgcttttg cttttcggct
2161 ccatgcaatg gccctcggag agcgcaccgc ccgaagggtg gccgttaggc cagtttctcg
2221 aagagaaacc ggtaagtgcg ccctccccta caaagtaggg tcgggattgc cgccgctgtg
2281 cctccatgat agcctacgag acagcacatt aacaatgggg tgtcaagatg gttaagggga
2341 gcaacaaggc ggcggatcgg ctggccaagc tcgaagaaca acgagcgcga atcaatgccg
2401 aaattcagcg ggtgcgggca agggaacagc agcaagagcg caagaacgaa acaaggcgca
2461 aggtgctggt gggggccatg attttggcca aggtgaacag cagcgagtgg ccggaggatc
2521 ggctcatggc ggcaatggat gcgtaccttg aacgcgacca cgaccgcgcc ttgttcggtc
2581 tgccgccacg ccagaaggat gagccgggct gaatgatcga ccgagacagg ccctgcgggg
2641 ctgcacacgc gcccccaccc ttcgggtagg gggaaaggcc gctaaagcgg ctaaaagcgc
2701 tccagcgtat ttctgcgggg tttggtgtgg ggtttagcgg gctttgcccg cctttccccc
2761 tgccgcgcag cggtggggcg gtgtgtagcc tagcgcagcg aatagaccag ctatccggcc
2821 tctggccggg catattgggc aagggcagca gcgccccaca agggcgctga taaccgcgcc
2881 tagtggatta ttcttagata atcatggatg gatttttcca acaccccgcc agcccccgcc
2941 cctgctgggt ttgcaggttt gggggcgtga cagttattgc aggggttcgt gacagttatt
3001 gcaggggggc gtgacagtta ttgcaggggt tcgtgacagt tagtacggga gtgacgggca
3061 ctggctggca atgtctagca acggcaggca tttcggctga gggtaaaaga actttccgct
3121 aagcgataga ctgtatgtaa acacagtatt gcaaggacgc ggaacatgcc tcatgtggcg
3181 gccaggacgg ccagccggga tcgggatact ggtcgttacc agagccaccg acccgagcaa
3241 acccttctct atcagatcgt tgacgagtat tacccggcat tcgctgcgct tatggcagag
3301 cagggaaagg aattgccggg ctatgtgcaa cgggaatttg aagaatttct ccaatgcggg
3361 cggctggagc atggctttct acgggttcgc tgcgagtctt gccacgccga gcacctggtc
3421 gctttcagaa atcaatctaa agtatatatg agtaaacttg gtctgacagg cccctgaatt
3481 cgcatctaga ctgatgagac gtggtagagc cacaaacagc cggtacaagc aacgatctcc
3541 aggaccatct gaatcatgcg cggatgacac gaactcacga cggcgatcac agacattaac
3601 ccacagtaca gacactgcga caacgtggca attcgtcgca atacaacgga cagtcattca
3661 tctttctgcc cctccaaaag caaaaacccg ccgaagcggg tttttacgta aatcaggtga
3721 aactgaccga ctttggcagt ttattcttga catgtagtga gggggctggt ataatcacat
3781 agtactgttt atgagtaaag gagaagagct tttcacagga gttgtcccaa tcctcgtgga
3841 attagacggt gatgttaatg ggcacaagtt ctctgtcagt ggagagggtg aaggcgacgc
3901 aacatatggc aagctgaccc ttaaatttat ttgcaccacg ggtaaactac ctgttccatg
3961 gccaacactg gtcactacgt tcgggtatgg ggttcagtgc tttgcgcgct acccagatca
4021 catgaaacag cacgactttt tcaagagtgc aatgcccgaa ggctatgtac aggagagaac
4081 catctttttt aaggatgacg gcaactataa gacacgcgcc gaagtgaagt tcgagggtga
4141 tacccttgtt aatagaatcg agttaaaggg tattgacttt aaggaagatg gaaatatttt
4201 aggccacaaa ctggaatata actataactc ccataatgtg tacattatgg ccgacaagca
4261 aaagaacggt atcaaggtta acttcaagat cagacacaac attgaggatg gaagcgttca
4321 actagccgac cattaccaac aaaacacccc aattggcgat gggcctgtgc tgttaccaga
4381 caaccattac ctgtccactc aatctgccct ttcgaaagat cccaacgaaa agcgcgacca
4441 catggtcctt cttgagtttg tcacggctgc tgggattaca cacggcatgg atgaactata
4501 caaataaatc caacagtact atgtgattat accagccccc tcactacatg tcaagaataa
4561 actgccaaag gtaatcgtta atccgcaaat aacgtaaaaa cccgcttcgg cgggtttttt
4621 tatgggggga gtttagggaa agagcatttg tcagctgcgt ctcaagcagt tacagagatg
4681 ttacgaaccc ccaggacatc cgagaatgcg aggcgatgga gggtacaacc cgagcggccg
4741 cttatttgcc gactaccttg gtgatctcgc ctttcacgta gtggacaaat tcttccaact
4801 gatctgcgcg cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa
4861 gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct
4921 tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat
4981 ttcgctcatc gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg
5041 cctcaaatag atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca
5101 aggcaacgct atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg
5161 ctggctcgaa gatacccgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc
5221 gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag
5281 cgcggagaat ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag
5341 ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt
5401 gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt
5461 cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca
5521 ccgcttccct cataatgttt aactttgttt tagggcgact gccctgctgc gtaacatcgt
5581 tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc
5641 gaggcataga ctgtacccca aaaaaacagt cataacaagc catgaaaacc gccactgcgc
5701 cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact
5761 tgcattacag cttacgaacc gaacaggctt atgtccactg ggttcgtgcc ttcatccgtt
5821 tccacggtgt gcgtcacccg gcaaccttgg gtagcagcga agtcgaggca tttctgtcct
5881 ggctggtcat gaccaaaatc ccttaacgtg agtcagcctg ccgccttggg ccgggtgatg
5941 tcgtacttgc ccgccgcgaa ctcggttacc gtccagccca gcgcgaccag ctccggcaac
6001 gcctcgcgca cccgctggcg gcgcttgcgc atggtcgaac cactggcctc tgacggccag
6061 acatagccgc acaaggtatc tatggaagcc ttgccggttt tgccggggtc gatccagcca
6121 cacagccgct ggtgcagcag gcgggcggtt tcgctgtcca gcgcccgcac ctcgtccatg
6181 ctgatgcgca catgctggcc gccacccatg acggcctgcg cgatcaaggg gttcagggcc
6241 acgtacaggc gcccgtccgc ctcgtcgctg gcgtactccg acagcagccg aaacccctgc
6301 cgcttgcggc cattctgggc gatgatggat accttccaaa ggcgctcgat gcagtcctgt
6361 atgtgcttga gcgccccacc actatcgacc tctgccccga tttcctttgc cagcgcccga
6421 tagctacctt tgaccaccat ggcatcagcg gtgacggcct cccacttggg ttccaggaac
6481 agccggagct gccgtccgcc ttcggtcttg ggttccgggc caagcactag gccattaggc
6541 ccagccatgg ccaccagccc ttgcaggatg cgcagatcat cagcgcccag cggctccggg
6601 ccgctgaact cgatccgctt gccgtcgccg tagtcatacg tcacgtccag cttgctgcgc
6661 ttgcgctcgc cccgcttgag ggcacggaac aggccggggg ccagacagtg cgccgggtcg
6721 tgccggacgt ggctgaggct gtgcttgttc ttaggcttca ccacggggca cccccttgct
6781 cttgcgctgc ctctccagca cggcgggctt gagcaccccg ccgtcatgcc gcctgaacca
6841 ccgatcagcg aacggtgcgc catagttggc cttgctcaca ccgaagcgga cgaagaaccg
6901 gcgctggtcg tcgtccacac cccattcctc ggcctcggcg ctggtcatgc tcgacaggta
6961 ggactgccag cggatgttat cgaccagtac cgagctgccc cggctggcct gctgctggtc
7021 gcctgcgccc atcatggccg cgcccttgct ggcatggtgc aggaacacga tagagcaccc
7081 ggtatcggcg gcgatggcct ccatgcgacc gatgacctgg gccatggggc cgctggcgtt
7141 ttcttcctcg atgtggaacc ggcgcagcgt gtccagcacc atcaggcggc ggccctcggc
7201 ggcgcgcttg aggccgtcga accactccgg ggccatgatg ttgggcaggc tgccgatcag
7261 cggctggatc agcaggccgt cagccacggc ttgccgttcc tcggcgctga ggtgcgcccc
7321 aagggcgtgc aggcggtgat gaatggcggt gggcgggtct tcggcgggca ggtagatcac
7381 cgggccggtg ggcagttcgc ccacctccag cagatccggc ccgcctgcaa tctgtgcggc
7441 cagttgcagg gccagcatgg atttaccggc accaccgggc gacaccagcg ccccgaccgt
7501 accggccacc atgttgggca aaacgtagtc cagcggtggc ggcgctgctg cgaacgcctc
7561 cagaatattg ataggcttat gggtagccat tgattgcctc ctttgcaggc agttggtggt
7621 taggcgctgg cggggtcact acccccgccc tgcgccgctc tgagttcttc caggcactcg
7681 cgcagcgcct cgtattcgtc gtcggtcagc cagaacttgc gctgacgcat ccctttggcc
7741 ttcatgcgct cggcatatcg cgcttggcgt acagcgtcag ggctggccag caggtcgccg
7801 gtctgcttgt ccttttggtc tttcatatca gtcaccgaga aacttgccgg ggccgaaagg
7861 cttgtcttcg cggaacaagg acaaggtgca gccgtcaagg ttaaggctgg ccatatcagc
7921 gactgaaaag cggccagcct cggccttgtt tgacgtataa ccaaagccac cgggcaacca
7981 atagcccttg tcacttttga tcaggtagac cgaccctgaa gcgctttttt cgtattccat
8041 aaaaccccct tctgtgcgtg agtactcata gtataacagg cgtgagtacc aacgcaagca
8101 c
//
